Quercetin directed transformation of calcium carbonate into porous calcite and their application as delivery system for future foods

The hierarchically porous property of CaCO3 has attracted considerable attention in the field of active delivery ingredients due to its high adsorption capacity. Here, a facile and high-efficient approach to control the calcification processes of CaCO3 ending with calcite microparticles with superior porosity and stability is reported and evaluated. In this work, a series of quercetin promoted CaCO3 microparticles, using soy protein isolate (SPI) as entrapment agent, was synthesized, characterized, and their digestive behavior and antibacterial activity were evaluated. Results obtained indicated that quercetin showed good ability to direct the calcification pathway of amorphous calcium carbonate (ACC) with the formation of flower- and petal-like structures. The quercetin-loaded CaCO3 microparticles (QCM) had a macro-meso-micropore structure, which was identified to be the calcite form. The macro-meso-micropore structure provided QCM with the largest surface area of 78.984 m2g-1. The loading ratio of SPI to QCM was up to 200.94 μg per mg of QCM. The protein and quercetin composite microparticles (PQM) were produced by simply dissolving the CaCO3 core, and the obtained PQM was used for the delivery of quercetin and protein. Thermogravimetric analysis showed PQM presented with good thermal stability without the CaCO3 core. Furthermore, minor discrepancy was noted in protein conformational structures after removing the CaCO3 core. In vitro digestion revealed that approximately 80% of the loaded quercetin was released from PQM during intestinal digestion, and the released quercetin exhibited efficient transportation across the Caco-2 cell monolayer. More importantly, the PQM digesta retained enhanced antibacterial activities to inhibit growth of Escherichia coli and Staphylococcus aureus. Porous calcites show a high potential as a delivery system for food applications.