辐射敏感性
癌症研究
细胞凋亡
癌症
细胞周期
MAPK/ERK通路
体内
衰老
受体酪氨酸激酶
蛋白激酶B
细胞生长
癌细胞
细胞周期检查点
PI3K/AKT/mTOR通路
生物
信号转导
化学
细胞生物学
放射治疗
医学
内科学
生物化学
生物技术
遗传学
作者
Qian Niu,Shiyuan Liu,Ting‐Ting Huang,Beibei Su,Yuanyuan Zhang,Xianglin Yuan
出处
期刊:Oncology Research and Treatment
[S. Karger AG]
日期:2022-12-08
卷期号:46 (1-2): 11-25
被引量:1
摘要
Background: Radiotherapy has an unsatisfactory effect on gastric cancer. The purpose of this study was to investigate the effect of pyrotinib, a highly effective human epidermal growth factor receptor (HER) family inhibitor, on the radiosensitivity of HER2-positive gastric cancer and its mechanism in vivo and in vitro. Methods: NCI-N87 and SNU-216 were HER2-positive gastric cancer cell lines; these cell lines were treated with or without 0.01 μM pyrotinib 12 h before irradiation. The proliferation capacity was determined by CCK8, and clone formation experiments were used to test the radiosensitization effect of pyrotinib. The expression of HER2, γ-H2AX, apoptosis protein, senescence-associated proteins, and AKT/ERK signaling pathway changes were determined by Western blot. Cell cycle and apoptosis were established by flow cytology. Immunofluorescence was utilized to detect the expression of HER2 and γ-H2AX. Senescence cells were stained by β-galactosidase staining method. Pathway enrichment analysis was tested by RNA sequencing. Next, a xenograft tumor model was constructed in nude mice for verification in vivo. Results: The clone formation experiment indicated the radiosensitivity of pyrotinib. The combined treatment can inhibit the entry of HER2 protein into the nucleus and reduce the phosphorylation of the ERK signaling pathway caused by irradiation. Pyrotinib also enhances DNA damage; induces apoptosis, G2/M phase arrest; and promotes senescence. In the xenograft model, the tumor inhibition rate was significantly stronger in the combined treatment group. Conclusions: Our results demonstrated that pyrotinib can induce radiosensitivity in HER2-positive gastric cancer in vivo and in vitro. This effect is through reducing irradiation-induced HER2 entry into the nucleus and inhibiting ERK1/2 signaling pathway.
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