Isolation of bacteria and yeast from blood using antibody-conjugated magnetic beads

Rapid detection of bloodstream infection pathogens is essential, as traditional blood cultures require 24-72 hours, delaying treatment and reducing survival rates. This study aims to develop a simple, efficient strategy for rapidly isolating Pseudomonas aeruginosa (Gram-negative), Staphylococcus aureus (Gram-positive), and Candida albicans (yeast) directly from blood while preserving whole-cell integrity for downstream applications like fluorescence staining. The method employs antibody-conjugated magnetic beads to capture pathogens directly from blood, enhancing detection sensitivity for low-abundance organisms. Pathogens are eluted as intact cells, enabling visualization via fluorescence staining. The approach successfully isolated Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, demonstrating robust performance and clinical potential. Antibody-conjugated magnetic beads offer a rapid, sensitive, and efficient solution for pathogen isolation from blood, maintaining cell integrity for further analysis. This method improves detection and diagnosis of BSIs, supporting timely interventions and better patient outcomes.