Analysis and validation of diagnostic biomarkers and immune cell infiltration characteristics in osteoarthritis by integrating bioinformatics and machine learning

The results of screening Hub OA-ERDEGs using machine learning algorithms show that: TOLLIP, ALB, HP, RHOBTB3, GSTM2, S100A8 and AKR1B1 were significantly up-regulated or down-regulated in OA samples and verified by qRT- PCR for validation. Using the ssGSEA algorithm, we discovered that 8 types of immune cell infiltration and 5 types of immune cell activation.