化学
血红素蛋白
血红素
基质(水族馆)
吸收光谱法
光谱学
细胞色素P450
铁质
吸收(声学)
细胞色素
分光光度法
一氧化碳
光化学
立体化学
分析化学(期刊)
色谱法
有机化学
酶
材料科学
物理
地质学
复合材料
催化作用
海洋学
量子力学
作者
John B. Schenkman,Ingela Jansson
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2006-06-30
卷期号:: 11-18
被引量:8
标识
DOI:10.1385/1-59259-998-2:11
摘要
UV/Vis spectroscopy is the major means of identifying intact holocytochrome P450. The carbon monoxide complex of the intact ferrous hemoprotein exhibits a characteristic spectrum between 448 and 452 nm, considerably distinct from the usual Soret absorption peaks of hemoproteins. Methods are described for identification and quantitation of cytochrome P450 (CYP) in membranes, in tissue homogenates, and in purified form, using difference spectroscopy and absolute spectroscopy. CYP are b-type cytochromes, containing protoporphyrin IX as the prosthetic group. Methods are also provided, using alkali and pyridine, for quantitation of the hemoprotein by this prosthetic group. In its oxidized, or ferric state, CYP exists as an equilibrium mixture of high- and low-spin configurations, each with distinctive UV/Vis absorption peaks. Substrate binding causes shifts in the spin equilibrium, and methods are shown for using these shifts for quantitation of substrate binding to CYP.
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