Soil seedbank: Importance for revegetation in the water level fluctuation zone of the reservoir area

Vegetation succession in the water level fluctuation zone (WLFZ) is driven by periodical water fluctuations, the mechanisms of response and synergistic evolution between aboveground vegetation (AGV) and soil seed bank (SSB) in the WLFZ remain unclear. To illustrate the response between AGV and SSB and the importance of SSB for revegetation in the WLFZ, the Three Gorges Reservoir (TGR) was taken as the target in the current study, and five sampling sites that lie in the center of reservoir and major tributaries of the TGR were selected. Plant community survey along the three water level gradients (i.e., 165-170 m, 170-175 m and 175-180 m) was conducted. Simultaneously, SSB samples on the topsoil (0-5 cm) were collected for germination experiments. A total of forty-nine species were observed from the SSB, belonging to 24 families and 47 genera, of which Asteraceae (8 species) and Poaceae (6 species) dominated. The number of total germinated seedlings species from soil samples from the sampling sites differed, i.e., was lower in Fengjie and Wushan compared to Yunyang, Gaoyang and Fengdu. The seed density in 165-170 m was significantly lower than that of 170-175 m and 175-180 m (p < 0.05). The Sørensen similarity coefficients between AGV and SSB tended to decrease with the increase of water level gradient, ranging from 0.04 to 0.42. SSB species composition was significantly associated with total carbon and total nitrogen contents of the soil (both p < 0.05). The SSB density was significantly negatively correlated with concentration of soil total nitrogen (p < 0.05), the species richness of SSB was significantly negatively correlated with soil pH value (p < 0.05). Hence, the relationship between the SSB and the soil habitat might be an important factor driving the construction of vegetation in the WLFZ. The correlation between dynamic of SSB and TGR hydrological regimes should be considered for revegetation in the WLFZ.