类有机物
上皮
细胞生物学
癌变
基质(化学分析)
生物
细胞培养
病理
干细胞
解剖
化学
癌症
医学
遗传学
色谱法
作者
Hiroko Hisha,Hikaru Ueno
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 93-99
被引量:5
摘要
A novel lingual epithelial organoid culture system using a three-dimensional (3D) matrix and growth factors has recently been established. In the culture system, organoids with multilayered squamous keratinized epithelium and typical morphological features of filiform papillae are generated from single lingual epithelial cells at a high efficiency. The culture system is created in order to observe the differentiation and maturation process of each lingual epithelial stem cell and to observe abnormal organoid formation from malignant cells obtained from carcinogen-treated mice. Thus, our culture system will contribute to the advancement of research into the regulatory mechanism of lingual epithelium and the underlying mechanisms of carcinogenesis.
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