The purpose of this study is to investigate changes in the forearm-muscle fatigue of a train driver operating the MasCon by using EMG (electromyogram) measurement technique. Train drivers usually use their forearm 4hours/day for normal operation. Accordingly, few different EMG signals of deltoid, biceps brachii, brachioradialis, flexor carpi ulnaris muscle of upper extremity have been measured and analyzed. The raw EMG Signals have been converted into median frequency using spectrum analysis. As the result, 80% of 10 subjects (real train drivers) showed that median frequency value of all four muscles has been reduced after 30 minutes of train operation. This results demonstrated that operating MasCon for 30 minutes could induce muscle fatigue.