生物
线粒体核糖体
核糖体
核糖体RNA
生物发生
甲基化
甲基转移酶
核糖体生物发生
核糖体蛋白
肽基转移酶
遗传学
细胞生物学
蛋白质亚单位
核糖核酸
基因
作者
Metodi D. Metodiev,Henrik Spåhr,Paola Loguercio Polosa,Caroline Meharg,Christian Becker,Janine Altmueller,Bianca Habermann,Nils‐Göran Larsson,Benedetta Ruzzenente
出处
期刊:PLOS Genetics
日期:2014-02-06
卷期号:10 (2): e1004110-e1004110
被引量:228
标识
DOI:10.1371/journal.pgen.1004110
摘要
Biogenesis of mammalian mitochondrial ribosomes requires a concerted maturation of both the small (SSU) and large subunit (LSU). We demonstrate here that the m(5)C methyltransferase NSUN4, which forms a complex with MTERF4, is essential in mitochondrial ribosomal biogenesis as mitochondrial translation is abolished in conditional Nsun4 mouse knockouts. Deep sequencing of bisulfite-treated RNA shows that NSUN4 methylates cytosine 911 in 12S rRNA (m5C911) of the SSU. Surprisingly, NSUN4 does not need MTERF4 to generate this modification. Instead, the NSUN4/MTERF4 complex is required to assemble the SSU and LSU to form a monosome. NSUN4 is thus a dual function protein, which on the one hand is needed for 12S rRNA methylation and, on the other hand interacts with MTERF4 to facilitate monosome assembly. The presented data suggest that NSUN4 has a key role in controlling a final step in ribosome biogenesis to ensure that only the mature SSU and LSU are assembled.
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