Two New Homo‐aro‐cholestane Glycosides and a New Cholestane Glycoside from the Roots and Rhizomes of Paris polyphylla var. pseudothibetica

Abstract Two new homo‐aro‐cholestane glycosides and a new cholestane glycoside, along with three known saponins, were isolated from the 95% EtOH extract of the roots and rhizomes of Paris polyphylla var. pseudothibetica. The structures of the new compounds were elucidated as 3 β ‐ O ‐{ α ‐ L ‐rhamnopyranosyl‐(1→4)‐ α ‐ L ‐rhamnopyranosyl‐(1→4)‐[ α ‐ L ‐rhamnopyranosyl‐(1→2)]}‐ β ‐ D ‐glucopyranosylhomo‐aro‐cholest‐5‐ene‐26‐ O ‐ β ‐ D ‐glucopyranoside (parispseudoside A, 1 ), 3 β ‐ O ‐ α ‐ L ‐rhamnopyranosyl‐(1→2)‐ β ‐ D ‐glucopyranosylhomo‐aro‐cholest‐5‐ene‐26‐ O ‐ β ‐ D ‐glucopyranoside (parispseudoside B, 2 ), and (25 R )‐3 β ‐ O ‐{ α ‐ L ‐rhamnopyranosyl‐(1→4)‐ α ‐ L ‐rhamnopyranosyl‐(1→4)‐[ α ‐ L ‐rhamnopyranosyl‐(1→2)]}‐ β ‐ D ‐glucopyranosyl‐cholesta‐5,17(20)‐diene‐16,22‐dione‐26‐ O ‐ β ‐ D ‐glucopyranoside (parispseudoside C, 3 ) by spectroscopic methods, including 1D‐ and 2D‐NMR, and MS experiments, as well as chemical evidences.