γ-Glutamyl Transpeptidase in Isolated Brain Endothelial Cells: Induction by Glial Cells in Vitro

The endothelia of microvessels isolated from mouse brain by mechanical means are rich in γ-glutamyl transpeptidase; however, the enzyme often disappears when the cells migrate or proliferate from the microvessel isolates. In an endothelial cell line derived from similar isolates and negative for γ-glutamyl transpeptidase, the enzyme could be induced in the endothelial cells when they were cocultured with glial cells. Thus there may be a requirement for continuous induction of γ-glutamyl transpeptidase in brain microvessels by adjacent glial cells.