Influence of oligosaccharides on the antioxidant activities and tolerance capacity of Lactobacillus to oxidative stress

Objective:To evaluate the effects of galacto-oligosaccharide(GOS) and xylo-oligosaccharide(XOS) on the antioxidant activity and tolerance capacity of Lactobacillus under H2O2 stress. Methods:Under H2O2 stress,the survival of Lactobacillus was examined by MTT method and the integrity of cell membrane was examined by flow cytometry. The effects of GOS and XOS on the activity of antioxidant enzyme and expression of stress protein related gene in Lactobacillus were also evaluated. Results:Under the H2O2 stimulus,GOS and XOS could enhance the survival rate of Lactobacillus,which improved by 1.83～1.96 times. And the cell membrane integrity was increased by 17% and 13% respectively. Both oligosaccharides were able to enhance the activity of antioxidant enzyme and reduce the expression level of stress protein related gene in Lactobacillus. Conclusion:GOS and XOS are able to enhance the antioxidant activity and strengthen the resistance of Lactobacillus against oxidative stress.