Studied on Immunological Properties of Hantaan Virus G150.7 Recombinant Adenovirus

To investigate the immunological properties of recombinant adenovirus containing hantaan virus chimeric gene G1S0.7.Balb/c mice was directly immunized by recombinant adenovirus G1S0.7. The immune response was tested by ELISA, microcell-culture neutralizing experiment and T lymphocyte proliferation test (MTT assay). The results showed that the chimeric gene G1S0.7 can stimulate combining antibodies against NP (1:320) and GP (1:40) respectively detected by ELISA, which suggested that both GP and NP immune response were activated. The low-titer neutralization antibody reaction and clear lymphocyte proliferation were also detected simuleity. It is suggested that the recombinant adenovirus G1S0.7 can directly stimulate Balb/c mice to produce specific humoral immunity and cellular immunity to against hantaan virus.