吸附
化学
选择性吸附
聚合物
分子印迹聚合物
纳米材料
解吸
色谱法
选择性
二乙烯基苯
材料科学
有机化学
纳米技术
共聚物
催化作用
苯乙烯
作者
Lu Shi,Yuhai Tang,Yi Hao,Gaiyan He,Ruixia Gao,Xiaoshuang Tang
标识
DOI:10.1002/jssc.201600413
摘要
We report a core-shell magnetic molecularly imprinted polymer with high affinity through a facile sol-gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption-desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics.
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