Studies on the Nucleotides and Their Related Substances in Dried Laver

The perchloric acid extract of dried laver was fractionated by the procedure as shown in Fig. 1, and the fractions obtained were analyzed for nucleotides measuring Rf values in paperchromatography, absorption curves in ultraviolet region, and the others. Results were as follows. 1) Cytidylic acid, adenylic acid, uridylic acid and guanylic acid were conceived to be main nucleotides. Small amounts of di- or triphosphates, nucleosides and free bases were also detected. 2) It was ascertained that most of nucleotides in dried laver occurred in the form of 2'or 3'-nucleotides, the sugar component being ribose. 3) The contents of nucleotides was detemined to be 37-85 μmoles/g on the dry basis. It seems to be one of the foodstuffs richest in nucleotides.