Effect of Dahuang Fuzi decoction(大黄附子汤) on the expression of cytokines in severe acute pancreatitis rats

Objective: To study the effect of traditional Chinese medicine Dahuang Fuzi decoction(DHFZD,大黄附子汤) on the expression of cytokines in severe acute pancreatitis (SAP) rats. Methods: The SAP models of rats were constructed by inversively pouring of 1 5% sodium deoxycholate (1 ml/mg) through cholecystpancreatic duct in rats. Sixty male SD rats (260300 g) were divided into the control group, model group and DHFZD group randomly. The levels of amylase, tumor necrosis factorα(TNFα), interleukin1β (IL1β) and IL18 in serum were detected at different interval to explore the effect of DHFZD on cytokines expression. Results: Compared with the control group, the amylase, TNFα, IL1β and IL18 levels in serum in the model group were increased significantly and a positive correlation between the expression of amylase and TNFα, IL1β, IL18 was observed(all P 0 01). After the treatment with DHFZD , the levels of amylase, TNFα, IL1β and IL18 were decreased significantly compared with those of the model group(all P 0 01). Conclusion: DHFZD has prevention and treatment effect on SAP and downregulating the levels of TNFα,IL1β and IL18 in serum may be the mechanism.