Inhibition of gastric cancer cells invasion and metastasis by Darbufelone in vitro

Objective:To study the effects of Darbufelone on the invasion and metastasis of human gastric cancer cells SGC-7901 in vitro. Methods:The adhesion ability was determined by MTT.Invasion and metastasis was observed in Transwell Chamber membrane invasion culture system.The capability of SGC-7901 cell condition medium in inducing endothelial cell to form blood vessel in 24 well plate was detected.RT-PCR and cel1-immunohistochemistry were used to observe the expressions of OPN and MMP-2.Results:In the membrane invasion culture system,the numbers of invading and migrating SGC-7901 cells were significantly lower in Darbufelone treated groups compared with those in the control group(sP0.05).The vessel number and the length of vessel were significantly lower in Darbufelone treated groups compared with those in the control group(sP0.05).The adhesion rate,OPN and MMP-2 expression were down regulated in Darbufelone group(sP0.05).Conclusion:Darbufelone can inhibit the migration and invasion of SGC-7901 cells in vitro, probably via down-regulation of OPN and MMP-2.