Expression of COX-2 in A549 cells suppressed by selective COX-2 inhibitor Celecoxib

[Objective] This study reports whether a selective cox-2 inhibitor Celecoxib could inhibit the expression of cox-2 in A549 cell.[Methods] A549 cells were cultured in vitro with 5 ng/ml IL-1β and exposed to different concentrations Celecoxib.COX-2 mRNA was determined by RT-PCR.PGE2 was measured by ELISA.[Results] This study showed that different concentration Celecoxib suppress the expression of COX-2 in A549 cell and showed greater suppression at higher concentrations.[Conclusion] In this study,we demonstrate that a selective cox-2 inhibitor Celecoxib significantly suppress the expression of COX-2 in A549 cell and the effective suppress of gene is depend on the concentrations of Celecoxib.