Research on the Animal Model of Hepatic Fibrosis

Objective To find the best model of hepatic fibrosis.Methods Wistar rats were randomly allocated into three groups:pig serum group (n=30),subcutaneous injection carbon tetrachloride (CCl4) group (n=30) and intraperitoneal injection CCl4 group (n=30).Hepatic fibrosis was induced by subcutaneous,intraperitoneal injection of 40% CCl4 or pig serum (two times per a week) for 4 weeks and 6 weeks respectively in rats.Rat mortality,change of liver tissue and degree of hepatic fibrosis in all groups were examined.Results Hepatic fibrosis models were induced successfully by the three different methods,but rat mortalities in the pig serum group and 40% CCl4 subcutaneous injection group were significantly lower than in 40% CCl4 intraperitoneal injection group,and the time of hepatic fibrosis model formation in the 40% CCl4 group was shorter obviously than that in the other two groups.Conclusion Hepatic fibrosis induced by subcutaneous injection CCl4 is the best model of hepatic fibrosis.