Colloidal gold immunochromatographic test strips for broad-spectrum detection of Salmonella

Outbreaks of Salmonella infections have always attracted world-wide attention in food industry. Therefore, it is particularly important to detect Salmonella . In this study, we aimed to develop a rapid, simple, sensitive, and broad-spectrum colloidal gold immunochromatographic test strip (ICTS) for detecting Salmonella . The prepared monoclonal antibody (mAb) 1B4 was labeled with colloidal gold and used as the capture antibody, as it can pair with itself to form a sandwich detection platform. The sensitivity of the test strip for Salmonella typhimurium was 4 × 10 5 CFU/mL with naked eye and the pathogen was detected in 5–15 min. The colloidal gold ICTS can be used to detect 18 stains of Salmonella and there was no cross-reaction with 14 other food-borne pathogens. Salmonella typhimurium , Salmonella paratyphi B , and Salmonella enterica in chicken were detected after 7 h, 7 h and 6 h of incubation, respectively, and the detection limit was as low as 1 CFU/mL. In short, this is important reports of colloidal gold ICTS for detecting so many stains of Salmonella, and this will be a huge improvement for detecting multiple Salmonella in food samples. • The prepared monoclonal antibody 1B4 can pair with itself to form a sandwich detection platform. • The developed immunochromatographic test strip can detect 18 stains of Salmonella. • The sensitivity for Salmonella typhimurium detection is 4 × 10 5 CFU/mL. • The detection limit for three species of Salmonella in chicken is as low as 1 CFU/mL. • The detection time of this colloidal gold immunochromatographic test strips is less than 15 min.