生物
核酸
蜜蜂
核糖核酸
DNA
计算生物学
基因组DNA
养蜂女孩
生物技术
遗传学
生态学
基因
作者
Rohan Swami,Brooke Ganser,David R. Tarpy,Micheline K. Strand,Hongmei Li‐Byarlay
摘要
Abstract The honey bee is an excellent model system to study behavioral ecology, behavioral genetics, and sociogenomics. Nucleic acid-based analyses enable a broad scope of research in functional genomics, disease diagnostics, mutant screening, and genetic breeding. Multiple levels of analysis lead to a more comprehensive understanding of the causes of phenotypic variation by integrating genomic variation, transcriptomic profiles, and epigenomic information. One limitation, however, is the sample preparation procedures to obtain high quality DNA and RNA simultaneously, particularly from small amounts of material, such as tissues of individual bees. We demonstrate that it is feasible to perform dual extractions of DNA and RNA from a single individual bee and compare the quality and quantity of the extracted nucleic acids using two different types of methods. There was a greater total yield of DNA and RNA from ethanol-based extractions with minimal differences in overall concentration in ng/uL. We describe here the first validated method for dual extraction of DNA and RNA specifically from individual honey bees (Apis mellifera).
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