二氧乙烷
化学发光
近红外光谱
体内
分子成像
临床前影像学
聚集诱导发射
化学
荧光
纳米技术
材料科学
光学
物理
生物
色谱法
生物技术
作者
Jingsheng Huang,Yuyan Jiang,Jingchao Li,Jiaguo Huang,Kanyi Pu
标识
DOI:10.1002/anie.202013531
摘要
Abstract Chemiluminescence imaging is imperative for diagnostics and imaging due to its intrinsically high sensitivity. To improve in vivo detection of biomarkers, chemiluminophores that simultaneously possess near‐infrared (NIR) emission and modular structures amenable to construction of activatable probes are highly desired; however, these are rare. Herein, we report two chemiluminophores with record long NIR emission (>750 nm) via integration of dicyanomethylene‐4H‐benzothiopyran or dicyanomethylene‐4H‐benzoselenopyran with dioxetane unit. Caging of the chemiluminophores with different cleavable moieties produces NIR chemiluminescence probes (NCPs) that only produce signals upon reaction with reactive oxygen species or enzymes, for example, β‐galactosidase, with a tissue‐penetration depth of up to 2 cm. Thus, this study provides NIR chemiluminescence molecular scaffolds applicable for in vivo turn‐on imaging of versatile biomarkers in deep tissues.
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