荧光
电子舌
儿茶酚胺
多巴胺
化学
去甲肾上腺素
分析化学(期刊)
生物系统
生物物理学
色谱法
生物化学
生物
神经科学
物理
量子力学
品味
作者
Somayeh Jafarinejad,Arafeh Bigdeli,Mahmoud Ghazi‐Khansari,Pezhman Sasanpour,M. Reza Hormozi-Nezhad
标识
DOI:10.1021/acschemneuro.9b00537
摘要
Catecholamine neurotransmitters, specifically, dopamine (DA), epinephrine (EP), and norepinephrine (NE), are known as substantial indicators of various neurological diseases. Developing rapid detection methods capable of simultaneously screening their concentrations is highly desired for early clinical diagnosis of such diseases. To this aim, we have designed an optical sensor array using three fluorescent dyes with distinct emission bands and have monitored variations in their emission profiles upon the addition of DA, EP, and NE in the presence of gold ions. Because of the different reducing power of catecholamines, differently sized gold nanoparticles (GNPs) with different levels of aggregation were generated, resulting in different amounts of spectral overlap between the absorption band of the in situ generated plasmonic GNPs and the emission bands of the fluorescent dyes. These energy-transfer-based fingerprint profiles were used to discriminate the neurotransmitters by applying pattern recognition methods including linear discriminant analysis (LDA) and artificial neural networks (ANN) and to determine their concentration using multiple linear regression (MLR). Our proposed array also showed a good performance in the discrimination of DA, EP, and NE in complex biological media such as human urine.
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