化学
赭曲霉毒素A
适体
色谱法
整体式高效液相色谱柱
分子印迹聚合物
吸附
栏(排版)
赭曲霉毒素
分子印迹
高效液相色谱法
真菌毒素
选择性
分子生物学
有机化学
食品科学
工程类
生物
催化作用
结构工程
连接(主束)
作者
Haixia Lyu,Haoran Sun,Yimen Zhu,Jun Wang,Zenghong Xie,Jinxia Li
标识
DOI:10.1016/j.aca.2019.12.052
摘要
In this study, a double-recognized aptamer-molecularly imprinted monolithic column (Apt-MIP monolithic column) was prepared by introducing both aptamer and MIP to reduce non-specific adsorption. Its preparation parameters such as the time of photo-initiation, the dosage of photo-initiator and the concentration of aptamer were investigated in detail. The recovery ratios of ochratoxin A (OTA) to ochratoxin B (OTB) on Apt-MIP monolithic column, Apt monolithic column and MIP monolithic column were 116.1, 40.8 and 69, respectively. Even if the concentration of OTB was 10 times that of OTA, the recovery of OTB was only about 2.9%. Applied to beer samples, the prepared Apt-MIP monolithic column drastically resisted background adsorption and the high-specificity recognition for OTA was obtained with the recoveries of 95.5–105.9%. This work provided a simple and effective method to selectively identify OTA from complex samples.
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