This is the first study using enzymatic hydrolysis to prepare protein hydrolysates from Grass Turtle. Degree of hydrolysis was clearly affected by enzymatic hydrolysis conditions. Enzymatic hydrolysis process led to improve Grass Turtle protein hydrolysate properties. The highest protein solubility was 95.56% at pH 6 in protein hydrolysate obtained at DH 19.52%.Antioxidant activities were affected by molecular weights and amino acid profiles of protein hydrolysate.