弹性蛋白酶
腹主动脉瘤
灌注
主动脉
胰弹性蛋白酶
腹主动脉
基质金属蛋白酶
医学
动脉瘤
主动脉瘤
酶谱
内科学
内分泌学
病理
生物
外科
生物化学
酶
作者
Gorav Ailawadi,Jonathan L. Eliason,Karen J. Roelofs,Indranil Sinha,Kevin K. Hannawa,Eric P. Kaldjian,Guanyi Lu,Peter K. Henke,James C. Stanley,Stephen J. Weiss,Robert W. Thompson,Gilbert R. Upchurch
标识
DOI:10.1161/01.atv.0000143386.26399.84
摘要
It is hypothesized that a male predominance, similar to that in humans, persists in a rodent model of experimental abdominal aortic aneurysm (AAA) via alterations in matrix metalloproteinases (MMPs).Group I experiments were as follows: elastase perfusion of the infrarenal aorta was performed in male (M) and female (F) rats. At 14 days, aortas were harvested for immunohistochemistry, real-time polymerase chain reaction (PCR), and zymography. Group II experiments were the following: abdominal aorta was transplanted from F or M donors into F or M recipients. At 14 days, rodents that had undergone transplantation underwent elastase perfusion. In group III, male rats were given estradiol or sham 5 days before elastase perfusion. In group I, M rats had larger AAAs with higher frequency than did F rats. M rat aortas had more significant macrophage infiltrates and increased matrix metalloproteinase (MMP)-9 production and activity. In group II, M-to-M aortic transplants uniformly developed aneurysms after elastase perfusion, whereas F-to-F aortic transplants remained resistant to aneurysm formation. F aortas transplanted into M recipients, however, lost aneurysm resistance. In group III, estradiol-treated rats demonstrated smaller aneurysms and less macrophage infiltrate and MMP-9 compared with M controls after elastase.These data provide evidence of gender-related differences in AAA development, which may reflect an estrogen-mediated reduction in macrophage MMP-9 production.
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