下调和上调
小RNA
转染
细胞生长
细胞生物学
趋化因子
化学
分子生物学
污渍
细胞迁移
信号转导
癌症研究
基因
生物
细胞
免疫学
炎症
生物化学
作者
Ghasem Ghasempour,Vahid Pirhajati Mahabadi,Mohammad Shabani,Asghar Mohammadi,Fahimeh Zamani-Garmsiri,Abdollah Amirfarhangi,Mahdi Karimi,Mohammad Najafi
标识
DOI:10.1016/j.mvr.2021.104172
摘要
VSMC proliferation and migration pathways play important roles in plaque formation in the vessel stenosis and re-stenosis processes. The microRNAs affect the expression of many genes that regulate these cellular processes. The aim of this study was to investigate the effects of miR-181b, miR-204, and miR-599 on the gene and protein expression levels of hematopoietic cell kinase (HCK) in VSMCs.miR-181b, miR-204 were predicted for the suppression of HCK in the chemokine signaling pathway using bioinformatics tools. Then, the VSMCs were transfected by PEI-containing microRNAs. The HCK gene and protein expression levels were evaluated using RT-qPCR and Western blotting techniques, respectively. Moreover, the cellular proliferation and migration were evaluated by MTT and scratch assay methods.The miR-181b and miR-204 decreased significantly the HCK gene and (total and phosphorylated) protein expression levels. Also, the miR-599 did not show any significant effects on the HCK gene and protein levels. The data also showed that miR-181b, miR-204, and miR-599 prevent significantly the proliferation and migration of VSMCs.The downregulation of HCK by miR-181b and miR-204 suppressed the VSMC proliferation and migration.
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