枯草芽孢杆菌
采后
麦角甾醇
胶孢炭疽菌
杀菌剂
食品科学
生物化学
细菌
酶
转录组
炭疽菌
解淀粉芽孢杆菌
蛋白质组学
灰葡萄孢菌
化学
生物
黑曲霉
微生物学
抗真菌
菌丝体
代谢组学
发酵
孢子
作者
Ke Wang,Zhen Qin,Shiyuan Wu,Pengyu Zhao,Chaoying Zhen,Haiyan Gao
标识
DOI:10.1021/acs.jafc.1c00640
摘要
Bacillus subtilis is commonly used as a biocontrol bacterium owing to its strong antifungal activity, broad-spectrum inhibition, and general safety. In this study, the inhibitory effects of volatile organic compounds (VOCs) produced by B. subtilis CF-3 on Colletotrichum gloeosporioides, a major destructive phytopathogen of litchi anthracnose, were analyzed using proteomics and transcriptomics. Differentially expressed genes (DEGs) and proteins (DEPs) indicated that the inhibition of C. gloeosporioides by B. subtilis CF-3 VOCs downregulated the expression of genes related to cell membrane fluidity, cell wall integrity, energy metabolism, and production of cell wall-degrading enzymes. Particularly, those important DEGs and DEPs related to the ergosterol biosynthetic and biosynthesis of unsaturated fatty acids are most significantly influenced. 2,4-di-tert-butylphenol, a characteristic component of B. subtilis CF-3 VOCs, also showed a similar effect on C. gloeosporioides. Our results provide a theoretical basis for the potential application of B. subtilis CF-3 in the postharvest protection of fruits and vegetables.
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