Non-alkaloid constituents of Vinca major

The present study was designed to investigate the non-alkaloid compounds from the leaves and stems of Vinca major cultivated in Yunnan Province,China.The compounds were isolated using chromatographic techniques.The structures were elucidated by 1D-and 2D-NMR spectroscopic methods in combination with UV,IR,and MS analyses.The 1,1-diphenyl-2-picrylhydrazyl（DPPH）-scavenging activity of Compounds 1–7 were evaluated.One new iridoid glycoside（compound 1）,together with 11 known compounds,were isolated from Vinca major.Compounds 1,5,and 6 showed moderate DPPH-scavenging activity,with IC50 values being 70.6,32.8,and 62.2 μmol·L-1,respectively.In conclusion,compound 1 is a newly identified iridoid glycoside with moderate antioxidant activity.