Recent advances in chitosan-based layer-by-layer biomaterials and their biomedical applications

In recent years, chitosan-based biomaterials have been continually and extensively researched by using layer-by-layer (LBL) assembly, due to their potentials in biomedicine. Various chitosan-based LBL materials have been newly developed and applied in different areas along with the development of technologies. This work reviews the recent advances of chitosan-based biomaterials produced by LBL assembly. Driving forces of LBL, for example electrostatic interactions, hydrogen bond as well as Schiff base linkage have been discussed. Various forms of chitosan-based LBL materials such as films/coatings, capsules and fibers have been reviewed. The applications of these biomaterials in the field of antimicrobial applications, drug delivery, wound dressings and tissue engineering have been comprehensively reviewed. • Chitosan-based layer-by-layer (LBL) biomaterials • Driving forces used to produce chitosan-based films, fibers and capsules • Advances in the biomedical applications of chitosan-based LBL biomaterials • Challenges and possible solutions for developing chitosan-based LBL biomaterials