Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell

Endogenous H2O2 generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform. With PMA as the stimulus, the cell released a small amount of H2O2 and its mitochondrial membrane potential (MMP) decrease was smaller, compared with that on the PDMS substrates.