This paper contains two parts. The flrst one deals with proposal and preparing of temperature dependencies measurement of bio-material thermal properties. The thermal proper- ties of each matter are dependent on its own temperature. The measurement of heat conduction and speciflc heat of bio-material is described in this paper. Knowledge of these parameters is very important for the thermal processes computer simulation. However, these properties are very bad to flnd out. The bio-material will be used for a real experiment of the cryosurgery optimization. The second part of this paper deals with computer simulation of prepared measurement. Real measurement will be provided in close future and measured thermal dependencies of bio-material thermal properties will be used in next one simulation of cryosurgery. The main aim of this simulation is to flnd optimized parameters of cryosurgery. Results of simulation will be used in real experiment of cryosurgery optimization. The thermal conduction and the speciflc heat are two most important thermal properties of each matter for computer simulation. However these parameters could be very difierent in whole tem- perature range. Knowledge of these temperature dependencies is very important for correct results obtaining. For example see Figures 1(a) and (c) where temperature dependencies of stainless steel AISI 302 are shown. We need to know temperature dependencies of the bio-material thermal properties. These properties will be used for real experiment of cryosurgery optimization. We have to use two physical approaches for obtaining of all important data. The flrst one is calculation of thermal conductivity from known parameters of system, see equation (1) and flgure 1 b). It's necessary to have only small temperature gradient T1 iT2 about 1K for exact determination of the thermal conductivity by the temperature in 1 of ¢x. Q = k ¢ A ¢ T1 i T2 ¢x ) k =