Adsorption of BSA on Metal Loaded Monosize p(GMA) Microspheres

Immobilized metal ion affinity chromatography (IMAC) is an efficient method for the adsorption of histidinehaving proteins so poly(glycidyl methacrylate) [p(GMA)] monosize microspheres with an average diameter of 1.6 µm were produced by dispersion polymerization for adsorption of bovine serum albumin. Cu(II) ions were chelated on the poly(glycidyl methacrylate)-iminodiacetic acid [p(GMA)-IDA] microspheres to be used in bovine serum albumin adsorption studies in batch system. Characterizations of the microspheres were carried out by scanning electron microscopy (SEM), elemental analysis, Fourier transform infrared (FTIR) spectroscopy. The maximum adsorption capacity of the p(GMA)-IDA-Cu(II) microspheres was found to be 278.6 mg/g polymer at pH 5.0. The elution studies were performed by 1.0 M NaCl solution. The obtained results showed that the metal chelated p(GMA)-IDA-Cu(II) microspheres can be evaluated as an efficicent adsorbent for albumin adsorption.