化学
硫化氢
荧光
Knoevenagel冷凝
吸光度
半胱氨酸
共焦
光化学
分子
硫黄
生物化学
有机化学
色谱法
催化作用
物理
量子力学
酶
数学
几何学
作者
Feng‐Zao Chen,Deman Han,Yuan Gao,Heng Liu,Shengfu Wang,Fangyu Zhou,Kaibin Li,Siqi Zhang,Wujun Shao,Yanling He
出处
期刊:Talanta
[Elsevier]
日期:2018-09-01
卷期号:187: 19-26
被引量:29
标识
DOI:10.1016/j.talanta.2018.05.010
摘要
Hydrogen sulfide and biothiol molecules such as Cys, Hcy, and GSH play important roles in biological systems. Exploiting a probe to simultaneously detect and distinguish them is quite important. In this work, a versatile fluorescent probe that can simultaneously detect and discriminate Cys/Hcy and H2S is reported. The probe easily prepared by the Knoevenagel condensation of cyanoacetylindole with chlorinated phenyl-propenal possessed three potential sites that could react with biothiols and H2S. This probe also exhibited rapidity, high selectivity, and sensitivity for Cys/Hcy and H2S with distinct optical signal changes. The probe was able to display obvious fluorescence enhancement at 480 nm for Cys/Hcy and unique absorbance enhancement at 500 nm for H2S. We also demonstrated that the probe can be successfully applied to image Cys in MCF-7 cells suing a confocal fluorescence microscope.
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