Study on the determination of dexynivalenol in edible tissues of swine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

A method has been developed for the determination of dexynivalenol in edible tissues of swine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry(LC-MS/MS).The samples were extracted by pure water and purified by C18 solid phase extraction,and then analyzed by the LC-MS/MS after redissolved by 30% methanol.The chromatographic column was Phenomenex Luna 5 μ C18(2),and the flowing phase was comprised with methanol-0.1% formic acid solution(V/V=3∶ 7).The mass spectrometry was run with electrospray ion source and in positive ions multiple reaction monitoring mode for the quantitative and qualitative analyses.Limit of detection(LOD) was 25 μg·kg-1,and limit of quantitation(LOQ) was 50 μg·kg-1.The recoveries of dexynivalenol in edible tissues of swine ranged from 80.2% to 99.4% in 50,100 and 500 μg·kg-1 concentration levels.The relative standard deviation ranged from 2.47% to 9.47%.