Repeated Batch Biotransformation from Isoeugenol to Vanillin by Immobilized <i>Bacillus fusiformis</i> Cells in CSTR Reactor

Bacillus fusiformis CGMCC1347, screened from soil, was immobilized successfully to biotransform isoeugenol to vanillin. The optimal immobilization conditions were 2.5% sodium alginate, 10% cell density and Ca2+ 0.1 mol l-1. The optimal reaction conditions in a 2 L CSTR reactor with immobilized Bacillus fusiformis CGMCC1347 cells were isoeugenol 600 mL, immobilized cells 400 g, pH 3.5 dH2O 400 mL, 37 oC, 200 r min-1 shaking speed and 0.8 vvm air capacity. The average vanillin concentration reached 39.26 g l-1 after 6 times reuse of immobilized cells in 2 L CSTR.