Two New Spirostanol Saponins from Reineckia carnea

Abstract Two new spirostanol saponins, (1β,3β,5β,25S)‐spirostan‐1,3‐diol 1‐(β‐D‐xylopyranoside) (1) and (1β,3β,5β,25S)‐spirostan‐1,3‐diol 1‐[α‐L‐rhamnopyranosyl‐(1→2)‐β‐D‐fucopyranoside] (2), along with two known compounds, (1β,3β,5β,25S)‐spirostan‐1,3‐diol 1‐[α‐L‐rhamnopyranosyl‐(1→2)‐β‐D‐xylopyranoside] (3) and (1β,3β,4β,5β,25S)‐spirostan‐1,3,4,5‐tetrol 5‐(β‐D‐glucopyranoside) (4) were isolated from the whole plant of Reineckia carnea. The structures of the new steroids were determined by detailed analysis of their 1D‐ and 2D‐NMR spectra and chemical methods, and by comparison with spectral data of known compounds. Compounds 3 and 4 were isolated from the genus Reineckia for the first time.