生物
单核苷酸多态性
遗传学
单倍型
单链构象多态性
基因
基因型
多态性(计算机科学)
外显子
限制性片段长度多态性
人口
遗传关联
社会学
人口学
作者
Lili Bian,S.Z. Wang,Q.G. Wang,S. Zhang,Y.X. Wang,H. Li
标识
DOI:10.1111/j.1439-0388.2008.00739.x
摘要
Summary The insulin‐like growth factor 1 (IGF1) is essential for normal embryonic and postnatal growth in mammals. In this study, a total of 342 F 2 individuals, derived from Broiler crossing to Baier layer (Northeast Agricultural University Resource Population, NEAURP), were used to investigate the associations of haplotypes in the chicken IGF1 (cIGF1) gene with body weight traits. Primers for the 5′‐flanking, exon 3 and 3′‐flanking regions of cIGF1 were designed according to chicken genome database. Single nucleotide polymorphisms (SNPs) between parental lines were detected by sequencing, and PCR restriction fragment length polymorphism (PCR‐RFLP) and PCR single‐stranded‐conformation polymorphism (PCR‐SSCP) methods were used to genotype the SNPs in the population. Haplotypes were constructed with the three SNPs detected. The association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.‐366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken.
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