DNA Microarray Technology in the Evaluation of Weight Management Potential of a Novel Calcium-Potassium Salt of (—)-Hydroxycitric Acid

Quality and quantity of diet and nutrients are key factors of human health and disease prevention. Molecular diagnostics and cellular signaling play a fundamental role in the usefulness of novel nutraceuticals and functional foods. Increasing knowledge of the genes and molecules involved in the development of obesity is creating new methods of obesity regulation. Traditional herbal medicines may have some potential in weight management. Botanical dietary supplements often contain complex mixtures of phytochemicals that have additive or synergistic interactions. Evidence from numerous human and animal dietary studies has demonstrated the potential therapeutic effects of traditional herbal medicines in controlling obesity. We analyzed the effects of low-dose oral administration of calcium-potassium salt of (-)-hydroxycitric acid (HCA-SX) on the body weight and abdominal fat transcriptome in rats. HCA-SX restricted body weight gain in rats and lowered abdominal fat leptin expression. High-density microarray analysis of 9960 genes and ESTs present in the fat tissue identified a small set of specific genes sensitive to dietary HCA-SX. Mitochondrial/nuclear proteins necessary for fundamental support of the tissue were not affected by HCA-SX, further demonstrating its safety. Functional characterization of HCA-SX sensitive genes revealed that up-regulation of genes encoding serotonin receptors represents a distinct effect of HCA-SX on appetite suppression.