8-羟基-2'-脱氧鸟苷
检出限
脱氧鸟苷
色谱法
分析物
高效液相色谱法
化学
固相萃取
尿
萃取(化学)
DNA氧化
泌尿系统
生物标志物
DNA
DNA损伤
生物化学
医学
内科学
标识
DOI:10.1016/s1043-6618(02)00080-4
摘要
The compound 8-hydroxy-2'-deoxyguanosine (8-OHdG), one of the major oxidative DNA base products, is a commonly used biomarker of oxidative DNA damage. The routine measurement of 8-OHdG in biologic samples, and especially in urine, is inherently a challenging analytical problem due to the low levels of the analyte and the complexity of the sample. In this study, a simple and rapid procedure based on one step solid phase extraction (SPE) followed by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) analysis is described. The methodology proposed resulted in a recovery of 74.5+/-12% for urinary 8-OHdG with a detection limit of 5.0 microg l(-1).
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