An Electrochemical Sensor for Determination of Vitamin B2 and B6 Based on AuNPs@PDA-RGO Modified Glassy Carbon Electrode

Insufficient or excessive consumption of Vitamin B 2 (Vit B 2 ) and Vitamin B 6 (Vit B 6 ) can cause harmful effects on human health conditions, it is therefore very important to control their content in supplements and food products. In this work, we report on a simple, sensitive and selective electrochemical sensor based on gold nanoparticles (AuNPs) on glassy carbon electrode (GCE) modified by a hybrid of polydopamine and reduced graphene (PDA-RGO), for determination of Vit B 2 and Vit B 6 . The hybrid nanomaterial comprising PDA-RGO supported AuNPs is constructed through a one-step GCE modification process for electrochemical quantification of Vit B 2 and Vit B 6 in pharmaceutical formulations. Cyclic voltammetry result reveals that the effective response to Vit B 2 and Vit B 6 is according to the high electrical conductivity of AuNPs and the large surface area of PDA-RGO. Meanwhile, the sensor modified with a composite film shows a synergistic effect for catalyzing the analytes. Under optimized experimental conditions, these sensors show wide linear ranges of 0.02−60.0 μM and 0.03−600.0 μM, with the detection limits (LODs) of 0.0096 μM and 0.025 μM for Vit B 2 and Vit B 6 , respectively. Additionally, the constructed sensors have been applied for quantifying Vit B 2 and Vit B 6 in pharmaceutical formulations. Such an effective electrochemical platform could be potentially applied for analyzing various components for therapeutic drug monitoring.