Cloning and Sequence Analysis of cDNA Encoding Porcine Toll-like Receptor 7

In this study we cloned cDNA of Toll-like receptor 7(TLR7) gene from total RNA of mesenteric lymph nodes(MLN) in Neijiang pig by RT-PCR and RACE(rapid amplification of cDNA ends).Sequence analysis indicated that the porcine TLR7 cDNA cloned was 3 834 bp in length(GenBank accession number: EF469730) and the open reading frame encodes a deduced protein with 1 050 amino acids residues.The analysis of deduced amino acids sequence indicated that TLR7 is a typical type I transmembrane protein with a LRR-RI ectodomains and a TIR cytoplasmic domains,the common structural features of TLRs.The comparison of the deduced amino acids sequence of porcine TLR7 with those of cattle,dog,human,cat and mouse showed that the amino acids homology similarity were 90.8%,87.4%,84.9%,86.7% and 78.2%,respectively and the TLR7 is highly conserved among the different mammal species.