The recombined inbred lines(RILs) from the cross of 6044 and 01-35 were used for the genetic analysis of kernel configuration and kernel weight characters.The results indicated that the kernel configuration of RILs was normal distributed and varied differently.The kernel length(KL)/kernel thickness(KT),kernel width(KW)/kernel thickness,kernel length/kernel width,kernel length and kernel width had higher generalized hereditary.The principle component analysis indicated that KL,KL/KT,KW/KT influenced kernel weight greatly.The thousand kernel weight(TKW) very significantly positively correlated with kernel length,kernel width and kernel thickness,while significantly negatively correlated with KL/KW,KL/KT and KW/KT.By stepwise regression and path analysis,it was concluded that to improve TKW should coordinate the relations of kernel length,kernel width and kernel thickness,especially kernel thickness.