About 10%glycerol as the main by-product was produced in the process of biodiesel production. How to convert glycerol to chemicals with high values has been a hot issue.1,3-propanediol produced from glycerol is an efficient route to reduce the cost of biodiesel production with the large amount of biodicsel production. By far,1,3-propanediol is being produced using glycerol as material by chemical synthesis method in industrial scale.Recently,biotransformation of glycerol has been drawn more attention,because conventional chemical synthesis have problems of high pressure and high temperature,release of toxic intermediates.The study progress on preparation of 1,3-propanediol from glycerol by microbial fermentation was reviewed.