Hydrolysis of soybean isoflavone glycoside

By orthogonal test,the optimum conditions for soybean isoflavone glycoside to be hydrolyzed into isoflavone aglycon were found.The optimum reaction conditions of acid hydrolysis of soybean isoflavone glycoside were as follows:concentration of HCl 3 mol/L,temperature 80 ℃,time 180 min.Under the optimum conditions,the hydrolysis rate by HCl reached 81.31%.The optimum reaction conditions of enzymatic hydrolysis of soybean isoflavone glycoside were as follows:pH 6.0,temperature 38 ℃,time 90 min,enzyme dosage 0.9 mg(based on 50 mg soybean isoflavone glycoside).Under the optimum conditions,the hydrolysis rate by enzyme reached 82.54%.The effect of enzymatic hydrolysis was better than that of acid hydrolysis.