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Highly sensitive stool DNA testing of Fusobacterium nucleatum as a marker for detection of colorectal tumours in a Japanese population

核梭杆菌 结直肠癌 梭杆菌 腺瘤 内科学 胃肠病学 结直肠腺瘤 人口 医学 拟杆菌科 癌症 肿瘤科 生物 拟杆菌 细菌 遗传学 环境卫生 牙龈卟啉单胞菌 牙周炎
作者
Yutaka Suehiro,Kouhei Sakai,M. Nishioka,Shinichi Hashimoto,Taro Takami,Shingo Higaki,Yoshitaro Shindo,Shoichi Hazama,Masaaki Oka,Hiroaki Nagano,Isao Sakaida,Takahiro Yamasaki
出处
期刊:Annals of Clinical Biochemistry [SAGE Publishing]
卷期号:54 (1): 86-91 被引量:64
标识
DOI:10.1177/0004563216643970
摘要

Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional methods prevented further explorations. Therefore, we developed a droplet digital polymerase chain reaction (PCR) assay for detecting Fusobacterium nucleatum in stool and investigated its clinical utility in the management of colorectal tumours in a Japanese population. Methods Feces were collected from 60 healthy subjects (control group) and from 11 patients with colorectal non-advanced adenomas (non-advanced adenoma group), 19 patients with colorectal advanced adenoma/carcinoma in situ (advanced adenoma/carcinoma in situ (CIS) group) and 158 patients with colorectal cancer of stages I to IV (colorectal cancer group). Absolute copy numbers of Fusobacterium nucleatum were measured by droplet digital PCR. Results The median copy number of Fusobacterium nucleatum was 17.5 in the control group, 311 in the non-advanced adenoma group, 122 in the advanced adenoma/CIS group, and 317 in the colorectal cancer group. In comparison with that in the control group, the Fusobacterium nucleatum level was significantly higher in the non-advanced adenoma group, the advanced adenoma/CIS group and the colorectal cancer group. Conclusions This study illustrates the potential of stool DNA testing of Fusobacterium nucleatum by droplet digital PCR to detect individuals with colorectal tumours in a Japanese population.
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