Study on Bacteriostasis of Freezing-dried Shuanghuanglian Powder on Klebsiella Pneumoniae

Objective:To explore the bacteriostasis effect of freezing-dried Shuanghuanglian powder on Klebsiella pneumoniae.Methods:Based on the principle of liquid with different concentrations having their own number of bacteria,ESBLs positive SHV Klebsiella pneumoniae was selected to draw the time-turbidy curve by borth dilution method.Results:Klebsiella pneumoniae has different growth staus at different concentration of Shuanghuanglian.As far as the standard strain(ATCC 700603) of Klebsiella pneumoniae was concerned,freezing-dried Shuanghuanglian powder has no significant effect on the growth of bacteria when the concentration of Shuanghuanglian was below 12.50 mg/mL.However,the growth of the standard strain of Klebsiella pneumoniae was significantly inhibited when Shuanghuanglian concentration was at 12.50 mg/mL.For the clinical strains of Klebsiella pneumoniae,different concentration of Shuanghuanglian all had bacteriostasis effect on Klebsiella pneumoniae,and the bacteriostasis effect was enhenced with the increase of concentration.The growth of all kinds of bacteria was completely inhibited at the concentration of Shuanghuanglian at 50 mg/mL.Conclusion:Freezing-dried Shuanghuanglian powder has significant bacteriostasis effect on Klebsiella pneumoniae.