Improvement of Determination of Gastrodin in Gastrodia Elata Blume. by HPLC

Objective: To determine the content of gastrodin in three different habitats' Gastrodia elata Blume.Method: To determine the content of gastrodin in three different habitats' Gastrodia elata Blume by HPLC,on Agilent TC-C18 column,with Methylcyanide-0.05% phosphoric acid(4:96) as mobile phase,1.0mL/min as speed of flow,detection wavelength at 220nm.Result: The good linear correlation was obtained in the range 20-200μg.The average recovery of spots(n = 9) was 97.93 %(RSD = 1.49 %).The content of gastrodin in the Yunnan Zhaotong,the Hubei Yichang,the Shanxi Xi Town respectively was 0.164%,0.263%,0.418%.Conclusion: There were obvious differences in the content of gastrodin in three different habitats' Gastrodia elata Blume.The content of gastrodin in Yunan Zhaotong's Gastrodia elata Blume was the lowest.