Changes of Extracellular Enzyme Activity and Extracellular Polysaccharide During the Submerged Culture of Corilus versicolor
作者
Junming Wang,NW Sci
摘要
Dynamic variation of extracellular enzyme activity ,extracellular polysaccharide and other relative target during the submerged culture of Corilus versicolor were investigated. The results showed that the maxmum of hypha growth was 6.436 g/L, appeared in the 12th days .There was not significant change of pH of the fluid medium through the whole period of the cultivation. The maxmun of extracellular protein consistencey was 1.06 mg/mL, appeared in the 11th days. The peak of guaicaal oxidase enzyme production appeared in the 12th days, the maxmum enzyme activity was 713.2 u. The peak of enzyme production of polyphenol oxidase appeared twice. The first appeared in the 12th days, and its enzyme activity was 660.1 u. The second appeared in the 18th days, its enzyme activity was 1001.0 u . Extracellular polysaccharide appeared its maxmum in the 12th days, it was 23.70 mg/L.