Absorption Effect of Activated Charcoal to Hormones during Plant Tissue Culture

The absorption effect of activated charcoal to IAA,KT and 2,4-D during plant tissue culture were studied.The results showed that:(1) the absorption capability of activated charcoal to IAA and KT was much larger than to 2,4-D;(2) the absorption capability of different particle diameter of activated charcoal to the 3 kinds of plant hormones was almost the same;(3) when the time of absorption longer than 12h,the amounts absorbed of all the 3 kinds of hormones were increased obviously;(4) the absorbed amounts of all 3 kinds of hormones had close relationship with the amounts of used activated charcoal;(5)the addition order of activated charcoal,hormones and MS medium ingredient had no obvious effect on the amounts of 3 kinds of hormone absorbed.